Lentiviral particles for tranduction of mammalian cell lines with a green emitting luciferase transgene, for use in in vivo and other applications.
RediFect™ Green Renilla-Puromycin Lentiviral Particles are self-inactivating, recombination incompetent lentiviral particles carrying green emitting Renilla luciferase transgene under control of the stable UbC promoter. The luciferase transgene is fused to the puromycin resistance gene via T2A “self-cleaving” linker peptide for efficient coexpression with selection marker. The lentiviral particles are pseudotyped with G glycoprotein from Vesicular Stomatitis Virus (VSVG), allowing efficient transduction of a wide variety of mammalian cells including most cancer cell lines, primary, stem and non-dividing cells.
· Each vial contains 1 x 107 units/ml of lentiviral particles resuspended in 200 ul
· The packaged material provides sufficient number of lentiviral particles for transduction of at least one cell line.
We recommend using this product to transduce human cell lines, or murine cell lines that will be used in Nude and SCID mouse models only. An immune response may occur in syngeneic mouse models.
For laboratory use only. This product is intended for animal research only and not for use in humans.