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Opera Phenix Plus High-Content Screening System HH14001000

The Opera Phenix® Plus High Content Screening System is the premier confocal solution for today's most demanding high content applications. Drawing on over a decade of experience with the industry-leading Opera® and Opera® Phenix system, the Opera Phenix Plus is designed for high-throughput high-content assays, phenotypic screening, assays using complex disease models, such as live cells, primary cells and microtissues, and fast-response assays, such as Ca2+ flux.


Deeper Insights From Your Most Demanding Assays

The Opera Phenix Plus high-content screening system’s innovative optical design lets you generate richer information through extremely sensitive confocal imaging and at higher throughput than ever through simultaneous acquisition. Because spectral crosstalk is reduced to a minimum, it delivers speed without compromising sensitivity. With proven automated water immersion lenses, you’ll achieve higher throughput and richer content, making it the ideal high-content screening system for discriminating phenotypes and studying complex disease models. And with its liquid handling option and fast imaging frame rate, you can tackle fast-response assays such as calcium flux or cardiomyocyte beating.

Speed or Sensitivity – No Compromise

Proprietary Synchrony Optics combine a microlens enhanced Nipkow spinning disk with dual view confocal optics to separate fluorescence excitation and emission during simultaneous acquisition minimizing spectral crosstalk – for greater speed and higher sensitivity.

  • Up to four large format sCMOS cameras with simultaneous acquisition in up to four channels, so you can generate images of exceptional quality - faster than ever
  • Custom-designed high NA water immersion objectives to capture more photons and provide high image resolution even in thick samples
  • State-of-the-art sCMOS image sensors deliver low signal-to-background noise, a wide dynamic range and high resolution – ideal for sensitive and quantitative measurements at short exposure times
  • Spinning microlens disk with increased pinhole-to-pinhole distance to further reduce out-of-focus noise in thick samples such as microtissues
  • Spinning disk optics and precise synchronization of the excitation laser and camera to minimize phototoxicity and bleaching for live cell assays
  • Widefield or confocal imaging modes depending on your assay requirements
  • Fast laser-based autofocus to ensure sharp confocal images from every field
  • Modular design to adapt the system to your changing application needs – choose from single, dual or four camera configuration with four solid state lasers or a five laser system optimized for FRET applications

Capture Fast Cellular Responses

The system’s imaging frame rate of up to 100fps is well-suited to assays that measure the beat-rate of cultured cardiomyocytes, as often used in cardiotoxicity studies. And the fast imaging frame rate, together with the dispense and read capabilities of the optional pipettor module, enables fast response assays in which cell responses occur within milliseconds to seconds – notably calcium flux assays.

  • Tip-based pipettor compatible with PerkinElmer 20µl and 200µl tips, 1-200µl in 1µl increments
  • Compound addition from one compound plate and one reservoir, up to 2 additions per well
  • Compatible with 96- and 384-well sample plates
  • Environmental control - temperature, CO2, and humidity
  • Compatible with automated exchange of sample plate, tip racks and compound plate
  • Liquid handling module is compatible with brightfield imaging

It All Comes Together in Harmony® – Acquisition to Analysis Made Easy

  • Intuitive workflow user-interface guides you through the entire process, from acquisition to analysis and evaluation
  • Templates for set-up of acquisition channels and parameters to get you productive right away
  • Ready-made solutions for common image analysis tasks so you can simply select and go
  • Image analysis building blocks that allow you to create, configure, and customize your own high content analysis applications
  • Advanced analysis features such as texture and STAR morphology analysis for detailed descriptions of cellular morphology and robust differentiation of cellular phenotypes
  • Data management for automatic storage of analysis results and metadata, such as assay layout, instrument, settings, plus user-defined keywords, and annotations

Your Total Solution for High Content Screening

  • Get better results from your Opera Phenix Plus system with our for high content screening, such as the CellCarrier Ultra 384-well microplates designed for optimal performance in high content imaging applications and our CellCarrier Spheroid ULA plates for imaging 3D models.
  • Improve throughput and productivity, and reduce variability and reagent costs by automating your Opera Phenix system, for example with the cell::explorer automated workstation
  • Export your results automatically into the Columbus Image Data Storage and Analysis System, so you can access, reanalyze, store, and share image data from Opera Phenix Plus and other HCS systems across your organization
  • Export your results to High Content Profiler powered by TIBCO Spotfire®, and perform screening data analysis and validation, QC analyses, calculate reliable normalization, perform multivariate hit stratification and drug response profiling

Opera Phenix Plus Configurations




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