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Tootekood CP41

PhenoVue Nile Red Lipid stain is a lipophilic organic molecule commonly used for localization and quantification of intracellular lipid droplets. ​

PhenoVue Nile Red Lipid stain exhibits bright yellow fluorescence and is validated for use in imaging microscopy and high-content screening applications.

PhenoVue Nile Red Lipid stain exhibits:

  • maximum excitation wavelength of 512nm and maximum emission wavelength of 585 when complexed with triglycerides
  • maximum excitation wavelength of 552nm and maximum emission wavelength of 638nm when complexed with phospholipids

PhenoVue Nile Red Lipid stain is a lipophilic organic molecule that is almost nonfluorescent in water and polar solvents. In lipid-rich environments, Nile Red exhibits enhanced yellow fluorescence, as well as red fluorescence to a lesser extent.

Nile Red is commonly used for localization and quantification of intracellular lipid droplets which are ​involved in lipid synthesis, metabolism and transportation as well as protein storage and degradation or viral replication, all of which are related to pathophysiology, including dyslipidemia, obesity, lipodystrophy, diabetes, fatty liver diseases, or atherosclerosis.

Features

Numbers of Vials Per Unit2
Quantity or Volume Per Vial10mg (31.4µmoles)
FormPowder
Storage-20°C
Recommended Working Concentration200nM (0.008ng/mL)​
Maximum Excitation Wavelength

512 nm - complexed with triglycerides
552 nm - complexed with phospholipids*
(*as shown in SpectraViewer below)

Maximum Emission Wavelength585 nm - complexed with triglycerides
638 nm - complexed with phospholipids*
(*as shown in SpectraViewer below)
Common Filter SetCy3.5
Live Cell StainingYes
Fixed Cell StainingYes. See Product Information Sheet for more information
Equivalent Number of Microplates10900-32700​ x 96-well microplates
9090-32700​ x 384-well microplates
17040-40900 x 1536-well microplates

For research use only.

Spetsifikatsioonid

Spetsifikatsioonid

Dokumendid

PhenoVue-Brochure.pdf

PhenoVue Cellular Imaging Reagents Brochure

EN | 2,763 MB

Download

Phenovue-Reagents-and-Microplates.pdf

Reagents Overview

EN | 583,494 KB

Download

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