PhenoVue Fluor 555 - Concanavalin A is a fluorescent lectin which displays high affinity for glycoproteins and glycolipids present at the cellular membranes. It can be used for cellular membrane staining, particularly the endoplasmic reticulum. PhenoVue Fluor 555 - Concanavalin A exhibits bright green fluorescence and is validated for use in imaging microscopy and high-content screening applications. Part of Revvity's portfolio of cellular imaging reagents, PhenoVue Fluor 555 - Concanavalin A has a maximum excitation wavelength of 555 nm and a maximum emission wavelength of 570 nm.
Concanavalin A is a plant homotetrameric lectin known to activate the immune system or induce apoptosis and autophagy. Concanavalin A displays high affinity for α-mannopyranosyl and α-glucopyranosyl residues of glycoproteins and glycolipids present at the cellular membranes. Fluorescent Concanavalin A derivatives are commonly used for staining the cellular membranes of mammalian cells, particularly the endoplasmic reticulum. PhenoVue Fluor 555 - Concanavalin A can be used to visualize cellular membranes in immunofluorescence, immunohistochemistry and flow cytometry, as well as high-content analysis and screening applications.
Features
| Numbers of Vials Per Unit | 5 |
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| Quantity or Volume Per Unit | 1mg (9.62nmoles) |
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| Form | Lyophilized |
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| Storage | 2 - 8°C |
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| Recommended Working Concentration | 100 µg/mL |
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| Maximum Excitation Wavelength | 555 nm |
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| Maximum Emission Wavelength | 570 nm |
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| Common Filter Set | Cy3 |
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| Live Cell Staining | Yes |
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| Fixed Cell Staining | Yes. See Product Information Sheet for more information |
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| Equivalent Number of Microplates | 1 - 5 x 96-well microplates
1 - 5 x 384-well microplates
3 - 8 x 1536-well microplates |
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Specifications
| Color | Yellow/Orange |
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| Detection Method | Fluorescence |
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| Filter | Cy3 |
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| Fluorophore | PhenoVue™ Fluor 555 |
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| Organelle or Cell Compartment | Plasma membranes, Endoplasmic Reticulum |
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| Product Compatibility | Live and fixed samples |
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| Product Brand Name | PhenoVue™ |
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| Shipping Condition | Ambient |
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| Type | Individual Reagent |
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| Unit Size | 5 vials |
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