Label antibodies, small molecules, proteins, or peptides with IVISense NIR fluorescent dyes.
Revvity’s IVISense™ fluorescent dyes (VivoTag®) for labeling antibodies, small molecules, proteins, or peptides are designed specifically to enable customized development of novel superbright fluorescent imaging agents. Dye properties, such as excellent brightness, hydrolytic stability, and photostability, as well as selection for minimal interaction with biological tissue for no potential loss of specificity, have been optimized and validated for use in in vivo or in vitro imaging.
Available as NHS esters or maleimide reactive dyes for conjugation to either free amine (-NH3) or free thiol (-SH) containing molecules. Conjugate an optimal 1-3 dyes onto your protein or choose self-quenching dyes to make your own custom fluorogenic probes.
IVISense 680 NHS fluorescent labeling kit is designed for preparing fluorescently labeled antibodies, proteins, or peptides for in vivo imaging applications in small animals. Each kit contains our superior in vivo optimized IVISense 680 NHS Fluorescent Dye fluorophore and everything else you need for carrying out the labeling reaction and purifying the labeled product. The reactive fluorophore has a succinimidyl ester group, which reacts with an amine group on the protein (i.e., lysine side-chain amine) to form a stable amide linkage. IVISense 680 NHS Fluorescent Dye is supplied in two separate vials, each containing adequate material for labeling 0.5-5 mg of an antibody. Following the labeling reaction, the unconjugated fluorophore is conveniently and rapidly removed by purification columns with a molecular weight cutoff of 7 kDa.
Contents:
- 2 X 0.25 mg of IVISense 680 NHS Fluorescent Dye
- 1 X 1 mL of 1M solution of sodium bicarbonate (pH8.3).
- 2 X Purification column
- 4 X 15 mL conical collection tube
- 1 X PBS (50 mL)
For research use only. Not for use in diagnostic procedures.
