Detect drug induced liver injury (DILI) early in the drug discovery process with Revvity' IVISense™ Toxicology Fluorescent Imaging Panel.
Drug-induced liver injury (DILI) is the leading reason for termination of drug discovery research projects and is a significant concern in attrition of new drug molecules reaching phase III clinical trials. DILI can manifest in hepatocellular, cholestatic, and steatotic forms differing in the type and pattern of tissue injury, biomarker expression and inflammation in the liver lobules.
Toxicology Fluorescent Imaging Panel of probes allows you to image inflammation, tissue destruction, metabolic changes, and vascular leak (edema), in a manner that can be predictive of potential drug toxicity, measuring biological changes prior to overt histological signs. Our published research shows the utility of a three-probe cocktail for imaging drug-induced liver injury (Vasquez & Peterson, J Pharmacol Exp Ther 2017; 361:87-98) via a ratio of probes specifically designed to optimize liver injury signal while minimizing normal background signal in liver and kidneys. The Toxicology Panel provides an extra vial of IVISense MMP 750 FAST to allow the preparation of the cocktail.
The inclusion of probes at 680 nm and 750 nm wavelengths further offers the opportunity for multiplex imaging of appropriate probe combinations to maximize information gained from research animals.
IVISense toxicology fluorescent imaging panel includes the following five probes (6 vials)
| Part number | Fluorescent probe | Biological target |
|---|
| NEV10054EX | IVISense Vascular 680 (AngioSense 680EX) | Vascular probe:
Leaks into sites of vascular damage or inflammation |
| NEV10091 | IVISense Transferrin Receptor 750 (Transferrin-Vivo 750) | Transferrin receptor targeted probe:
Iron metabolism marker in liver |
| NEV10168 x 2 | IVISense MMP 750 FAST (MMPSense 750 FAST) | Pan-MMP activatable probe:
Secreted marker of stellate cells, Kupffer cells, macrophages, and neutrophils |
| NEV11053 | IVISense Annexin-V 750 Fluorescent Probe (Annexin-Vivo) | Death/apoptosis probe:
Marker on inflammatory cells; metabolic marker on tumor cells |
| NEV11079 | IVISense Renin 680 FAST (ReninSense 680 FAST) | Renin activatable probe:
Marker of tissue renin-angiotensin system activity |
For research use only. Not for use in diagnostic procedures.
