Vivacon® 2 are centrifugal units for fast DNA concentration and buffer exchange of 2 ml samples. They are also the product of choice for protein fractionation applications. The use of Hydrosart® ultrafiltration membranes results in high recoveries from dilute samples. Complete recovery of the concentrate is guaranteed by a reverse spinning step.
Vivacon® 2 is optimized for the concentration / recovery and buffer exchange applications of genomic DNA and peptides for samples up to 2 ml initial volume. They are also the product of choice for peptide fractionation applications in this volume range. For optimal performance with highly diluted samples, e.g. forensic specimens, Vivacon® 2 is equipped with patented Hydrosart® regenerated cellulose membranes with extremely low unspecific binding properties and high flux. Selected MWCOs are available sterile and DNA free (PCR Grade units). They have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 106 and free of detectable genomic DNA contamination. Tests were performed with a qPCR assay over 55 cycles with a verified detection limit of 2 pg.
High recoveries and excellent reproducibility are paired with convenience offered by volume graduation and molecular weight cut-offs printed on individual devices. Vivacon® 2 features a horizontal membrane design optimal for DNA fractionation (primer removal) as well as peptide fractionation (Filter Aides Sample Preparation, FASP*). The possibility of a reverse spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations.
*Wisniewski JR, Zougman A, Nagaraj N, Mann M. Universal sample preparation method for proteome analysis (2009). Nat Methods. 6(5):359-62.
· Concentration of up to 2 ml genomic DNA or peptide samples
· Low binding material
· Reverse spin possibility
· MWCO printed on device
· Complete and highly reproducible concentrate recovery
· Selected cut offs available sterile and DNA free for e.g. forensic applications (qPCR assay over 55 cycles with a verified limit of detection < 2 pg)
