Corning® 100 mL HAT (Hypoxanthine, Aminopterin, Thymidine), 50x
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Tootekood 25-046-CI
Tootekood 25-046-CI
HAT Medium (hypoxanthine-aminopterin-thymidine medium) is used in mammalian cell culture for post-fusion selection to eliminate certain types of myeloma cells. The combination of aminopterin, a drug that acts as a powerful folate metabolism inhibitor, with hyproxanthine and thymidine, which are intermediates in DNA synthesis, provides a form of artificial selection for cells containing functioning hypoxanthine-guanine phosphoribosyltransferase (HGPRT) and thymidine kinase (TK). These enzymes are involved in DNA synthesis and cell division. HAT medium is often used in the preparation of monoclonal antibodies.
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Selection media
100 mL
1 / Pk
1 / Cs
-25°C to -15°C
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Tootekood 25-046-CI
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