Baker Co. invites you to join the webinars in this Autumn. First one will focus on Physoxia and new InvivO2 300 and second webinar for Microbiology/microbiome research and Bugbox Ax and Mx.
Making small changes for big impact - Paradigm shift from normoxia to physoxia
Date and time: Tuesday 24th of September, 2024 at 3PM EEST
Accurate life science experiments depend heavily on the quality and conditions of the materials used. Cells, which are sensitive to environmental variables like temperature, pH, and oxygen levels, can exhibit subtle, long-term effects from deviations from their optimal conditions. Oxygen, a critical molecule for cellular processes, must be maintained within a specific range to ensure accurate experimental outcomes. For instance, lung epithelial cells thrive in 10-14% oxygen, while neuronal cells require lower oxygen levels. Deviations from physiologically relevant oxygen levels can lead to misleading results. Constant hyperoxia, such as 18 % oxygen used in standard CO2 incubators likely provoke significant changes in cell signaling, including alterations in metabolism and gene expression. Thus, to obtain reliable data, it is crucial to simulate physiologically relevant oxygen conditions in cell culture environments, avoiding non-physiological stressors that could skew results.
Presented by Krista Rantanen Ph.D., Baker’s Director of Scientific Applications. Krista is a physoxia ambassador, hypoxia enthusiast, and champion for understanding the role oxygen plays in experimental biology.
Anything but oxygen – Considerations for anaerobic and microaerophilic bacteria culture
Date and time: Tuesday 1st of October, 2024 at 3PM EEST
The human intestine, known for its high regenerative capacity and low oxygen environment, is crucial for example nutrient absorption, waste removal, and immune regulation. The intestine epithelia have one of the steepest oxygen gradients in the body, ranging from highly vascularized sub-epithelial mucosa to near anaerobic lumen. The anaerobic environment in the lumen is largely created by high metabolic activity and oxygen consumption of epithelial cells and facultative bacteria, creating a home for the largest anaerobic bacteria population within the body. Thus, anaerobic bacteria are a significant part of human microbiota. Microbiome research focuses on behaviour, interaction, and function of microbiota in human physiology and aims to understand how the microbiota might affect certain pathologies. The Baker anaerobic and microaerophilic workstations facilitate research by providing controlled anaerobic or low oxygen conditions, crucial for studying the dynamics between microbiota and human tissue dynamics.
Presented by Krista Rantanen Ph.D., Baker’s Director of Scientific Applications. Krista is a physoxia ambassador, hypoxia enthusiast, and champion for understanding the role oxygen plays in experimental biology.
