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SKU 02202

APIS ESR1 Mutations Kit (RUO*)
The APIS ESR1 Mutations Kit is an advanced qPCR assay for the sensitive and precise detection of mutations within the oestrogen receptor gene. The APIS ESR1 Mutations Kit is a qualitative test, detecting eleven ESR1 mutations across three exons: exon 5 (E380Q), exon 7 (S463P) and exon 8 (P535H, L536R, L536Q, L536H, L536P, Y537C, Y537S, Y537N and D538G).
The APIS ESR1 Mutations Kit includes mutation-specific probes to enable highly sensitive detection of the target mutations. Additionally, it uses PCR clamp and blocker technology, which ensures specific amplification of the mutant sequence, even in the presence of a high wild-type background.

*APIS ESR1 Mutations Kit is for Research Use Only (RUO), not for use in diagnostic procedures.

Features of the Assay

Wide target coverage – the kit has been designed to cover 11 different ESR1 mutations
High specificity - includes clamp and blocking technology to ensure no wild-type detection
High sensitivity – all mutations are detected at ≤ 1% MAF (Mutant Allele Frequency)
Easy to use – our assays are designed to be user-friendly, with a simple protocol.
The reagents have been optimised for precise and sensitive detection in human DNA.

Specifications
Kit size: 24 Samples plus controls Specimen requirements:

  • 30 µl of DNA, e.g., extracted cell-free DNA (cfDNA).
  • It is recommended to extract samples using a cell-free-specific kit and to use the sample without dilution.

Turnaround time: 8 samples in <2 hours from PCR set up to results generation.

Additional information: https://www.biocartis.com/en/r...

Dokumendid

APIS-ESR1-RUO-Leaflet.pdf

EN | 288,535 KB

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APIS-ESR1-RUO-Technical-Sheet.pdf

EN | 241,158 KB

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