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Tootekood VN02H43

Vivacon® 2 are centrifugal units for fast DNA concentration and buffer exchange of 2 ml samples. They are also the product of choice for protein fractionation applications. The use of Hydrosart® ultrafiltration membranes results in high recoveries from dilute samples. Complete recovery of the concentrate is guaranteed by a reverse spinning step. Vivacon® 2 is optimized for the concentration / recovery and buffer exchange applications of genomic DNA and peptides for samples up to 2 ml initial volume. They are also the product of choice for peptide fractionation applications in this volume range. For optimal performance with highly diluted samples, e.g. forensic specimens, Vivacon® 2 is equipped with patented Hydrosart® regenerated cellulose membranes with extremely low unspecific binding properties and high flux. Selected MWCOs are available sterile and DNA free (PCR Grade units). They have been treated with a dual-cycle ethylene oxide (ETO) gas process to ensure they are sterile to a safety assurance level of 106 and free of detectable genomic DNA contamination. Tests were performed with a qPCR assay over 55 cycles with a verified detection limit of 2 pg. High recoveries and excellent reproducibility are paired with convenience offered by volume graduation and molecular weight cut-offs printed on individual devices. Vivacon® 2 features a horizontal membrane design optimal for DNA fractionation (primer removal) as well as peptide fractionation (Filter Aides Sample Preparation, FASP*). The possibility of a reverse spin after sample processing assures complete concentrate recovery which is especially important when working with low sample concentrations. *Wisniewski JR, Zougman A, Nagaraj N, Mann M. Universal sample preparation method for proteome analysis (2009). Nat Methods. 6(5):359-62. · Concentration of up to 2 ml genomic DNA or peptide samples · Low binding material · Reverse spin possibility · MWCO printed on device · Complete and highly reproducible concentrate recovery · Selected cut offs available sterile and DNA free for e.g. forensic applications (qPCR assay over 55 cycles with a verified limit of detection < 2 pg)

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Dokumendid

brochure-Ultrafiltration-Family.pdf

Sartorius® Ultrafiltration Family Brochure

EN | 1,611 MB

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Kontakt

Nova Natura UAB

Chemijos G. 11, LT-51347,
Kaunas, Lithuania

nova@novabio.lt
Phone: +370 (620) 70159

Reg No 305063819

Nova Natura OÜ

Kanali tee 6, 10112,
Tallinn, Estonia

nova@novabio.ee
Phone: +372 5656 0050

Reg No 14758203

Kontakt

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