Corning® Epidermal Growth Factor (EGF), mouse natural (receptor grade),500µg, is a low-molecular-weight mitogenic protein that stimulates proliferation of a wide variety of cell types in vitro. 100µg/container, 5/pack. These growth factors have been used to promote long-term proliferation in cell culture and to induce cell differentiation in a variety of cell types under serum-free or reduced serum conditions.
Formulation
Lyophilized from ammonium acetate, carrier-free; reconstitute in dH2O
Preparation and Storage
Stable for at least three months at 2° to 8°C (lyophilized) or =one month solubilized at -20°C . Do not store in frost-free freezer. Avoid multiple freeze/thaws.
Quality
Purity: >98% by SDS-PAGE and pH 9.9 PAGE
Tested for ability to stimulate proliferation of human foreskin fibroblasts in serum-containing medium
Filtered (0.2 µm membrane) and tested and found negative for bacteria, fungi, and mycoplasma
Source
Male mouse submaxillary glands
Molecular Weight
6.1 kD
Purification
EGF, mouse natural (receptor grade) - Purified by BioGel® P10/DEAE chromatography
Tip
Recommended concentration: Approximately 5 ng/ mL medium depending on cell type
Storage
Shelf life: >= 3 months from date of shipment at 2-8°C (lyophilized) or >= one month at -20°C (solubilized)
This product is non-returnable. If you have any questions, please contact us immediately.